Journal of Virology and Vaccine Development

Fast Track Diagnostic Requisite for Sepsis and its Antimicrobial Resistance in Low Resource Settings: A Step Towards Antimicrobial Stewardship

Dakhave Minal*, Amrita Khaire

R&D Department, Mylab Discovery Solutions Pvt. Ltd., Pune, Maharashtra, India

Abstract

Sepsis due to multidrug resistant bacteria (MDR) is a growing challenge. WHO developed the global action
plan on antimicrobial resistance and adopted a resolution for improving the prevention, diagnosis and clinical
management of the sepsis. At an early stage, sepsis frequently remains under-diagnosed when it is potentially reversible.
Laboratories in low resource settings often face the issue of poor infrastructure, irregular supply of reagents, maintenance
and new investment costs. Currently, molecular diagnostics have met the criteria of rapidity, ease of use, cost, automation
and can be used for point-of-care (POC) testing. However, there scarcity of POC diagnostics in the resource limited
settings. The present review describes real time polymerasebased tests and commercially available systems for their
capability for bacterial and resistance profile detection. We highlight the need for innovative, small and cost-effective
POC devices for clinical utility. It will help to plan more effective antibiotic treatments and quality of health care
services in developing countries.

Keywords:

Sepsis, bacterial pathogen, antibiotic resistance, molecular diagnostics, PCR