Journal of Virology and Vaccine Development

Viral Skin Disease in Angus Bull with Failure to Breed

Jing Huao*, Xi Yin, Wu Sheng

Department of Immunology, Beijing Institute of Technology, China

Abstract

Pseudocowpox, a Parapoxvirus, causes a typical delicate infection of the mamma and teats of cows. Infection of sex organ organs has not been reported before in bulls. during this report, pseudocowpox virus (PCPV) was known from one amongst 2 1-year-old Angus bulls (Bos taurus) with red rashes and hickey lesions on the surface of the erectile organ. immunization of bovine turbinate cells or primary bovine gonad cells with the swab of the hickey lesions made CPE characteristic of animal virus infection. Parapoxvirus-like particles were determined by EM in microorganism pellets of the tissue culture isolate. to verify the virus, selected 14V10296 to isolate, was a Parapoxvirus, microorganism DNA of the 14V10296 isolate was examined by PCR with pan Parapoxvirus primer sets that might amplify the B2L cistron of all four Parapoxviruses. The expected B2L amplicons of the 14V10296 isolate showed ninety-nine similarity to B2L of PCPV strain F00.120R. To more ensure the isolated virus is PCPV, PCR primers specific for Orf tube epithelium protein cistron and IL-10 and PCPV U DNA glycosidase (UDG) and IL-10, severally, were wont to amplify the microorganism DNA of the 14V10296 isolate. only if PCR primers specific to PCPV UDG and IL-10 were used, expected product were amplified from microorganism DNA of the 14V10296 isolate, severally. phyletic analysis suggests the 14V10296 isolate is closely associated with PCPV strain F00.120R, Associate in Nursing isolate from Greenland caribou. These results from tissue culture, EM, PCR amplification, and DNA sequence analysis counsel that the bulls were infected by a PCPV that is closely associated with PCPV strain F00.120R.

Keywords:

Angus bull, Pseudocowpox; Parapoxvirus.